Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

Degradation of active ingredient for herbicides sencor 60% SC (metribuzin) and turonex 50% SC (isoproturon) was determined by HPLC-DAD to study their resistance against decomposition when stored at zero temperature and under accelerated storage temperature when stored for 14 days at 54±2°C. According to FAO specification the physical properties for sencor and turonex herbicides such as suspensibility, pH values, pourability, persistent foam spontaneity of dispersion and wet sieve test were studied before and after the storage. Results illustrated that thermal storage at 54±2°C affected on metribuzin active ingredient more than isoproturon active ingredient which become conformity with FAO specification, but metribuzin become nonconformity with FAO specification after 10 days of storage. All physical properties for metribuzin and isoproturon formulations under thermal storage passed successfully except wet sieve test of metribuzin. On the other hand, some components of biochemical which carried out on albino rats were evaluated on the initial time at 23±2°C and at 54±2°C after 14 days of storage for both herbicides using 1/20 of their LD₅₀. The results at the initial time for both herbicides under the study showed highly decreasing in the content of total soluble protein with highly increasing in glucose, urea, creatinine, bilirubin, cholesterol and triglycerides levels.  Aspartate aminotransferase, Alkaline Phosphatase and Alanine Aminotransferase activities showed high results compared to control. Where, storage at 54±2°C leaded to slightly decreasing on content of total soluble protein, but bilirubin, serum glucose, cholesterol, urea, triglycerides and creatinine were low increase compared to health normal control.Activities of AST, ALT and ALP were the stimulated under the effect of the both herbicidescompared with the normal. Storage at 54±2°C until 14 days for both herbicides sencor 60% SC and turonex  was lower harmful than their effect at initial time at room temperature 23±2C°, the influences of metribuzin after thermal storage at 54±°C and at initial time 23±2°C on the biochemical study was more than that of isoproturon.