Detection of some Heavy Metals Using Rhodamine B Isothiocyanate Fluoresceinated Dye

Document Type : Original Article

Authors

1 Department of Chemistry, Faculty of Science, Suez Canal University.

2 Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University.

Abstract

The spectral behavior of the binary complexes of Rhodamine B Isothiocyanate (RBITC) dye with metal ions
in aqueous medium is studied. The observed fluorescence quenching of the dye reveals that the binding constant for
RBITC dye-metal ions follow the order Cu2+ > Ni2+ > Pb2+. Also, the spectral changes of the interaction of the studied
dye with metal ions in presence of ACES zwitterionic buffer (ternary complexes) is carried out, where the interaction of
dye with ACES zwitterionic buffer is accompanied by as light increasing of the peak intensity at λ= 585 nm and the
binding constant for dye-zwitterionic buffer- metal ion systems were determined where the order of binding with metal
ions is as follows: Pb2+ > Ni2+ > Cu2+.

Keywords