Antifungal Activity and Genetic Diversity of Selected Endophytic Fluorescent Pseudomonads

Document Type : Original Article

Authors

1 Department of Agricultural Botany, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

2 Department of Soil and Water, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

3 Department of Agricultural Microbiology, Faculty of Agriculture, Ain-Shams University, Cairo, Egypt

Abstract

Fluorescent Pseudomonads have been effectively utilized for controlling major fungal plant diseases because
of their antifungal metabolites. In this study, thirty-four bacterial isolates were isolated from root samples of different
crops and screened as potential biological control agents against Rhizoctoniasolani AG 2-2, Pythium arrhenomanes and
Fusarium solani. On the basis of dual culture assays, twenty isolates were selected for phenotypic characterization,
identification and plant growth promoting traits; four isolates were selected for greenhouse studies. The selected
Pseudomonas strains were characterized by PCR methods using rpob primer. Eight showed the ability to produce indole
acetic acid (IAA) while 12 isolates have no IAA produced, thirteen isolates could solubilize inorganic phosphate and six
strains produced hydrogen cyanide. Tested Pseudomonas strains had positive response on bean growth and health.
Obtained results indicated that the tested antagonistic fluorescent pseudomonads reduced disease severity in beans and
disease development under greenhouse pot experiment. However, further study is needed to apply to these results under
field conditions.

Keywords

Journal of Applied Plant Protection
Volume 4, Issue 1
December 2015
Pages 1-12

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