Characterization of Antifungal Metabolites from Antagonistic Fluorescent Pseudomonads

Document Type : Original Article

Authors

1 Department of Agricultural Botany, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

2 Department of Soil and Water, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

3 Department of Agricultural Microbiology, Faculty of Agriculture, Ain-Shams University, Cairo, Egypt

Abstract

Many Pseudomonas species produce antimicrobial metabolites for pathogen suppression and stimulate the
plant growth. So twenty fluorescent pseudomonas strains showed highest antagonism efficiency to soilborne
phytopathogens which used throughout the present investigation. These bacteria included five strains P. putida, 3/ P.
plecoglossicida, 3/ P. palleroniana, 2/ P. corrugate, 2/ P. entomophila and one from each of P. moraviensis, P.
parafulva, P. mosselii, P. anguilliseptica, P. argentinensis. Detection of the genes that encode for the production of
antibiotics by these strains such as phenazine-1-carboxylic acid (PCA), phenazine-1-carboxamide (PCN), pyrrolnitrin
(PRN), and pyoluteorin (PLT) was done by polymerase chain reaction (PCR) using gene-specific primers. Results
clearly showed that eight Pseudomonas strains produced (PCA), twelve strains produced (PCN), nine strains produced
(PLT) and two strains produced (PRN). Polymerase chain reaction (PCR) confirmed the obtained result by using TLC.
P. putida (SAW19) gave the highest antagonistic potential against tested phytopathogenic fungi and formulated using
different carriers to test for its viability on different carriers. The populations of P. putida were markedly higher in
vermiculite compared with the carriers under six months incubation period.

Keywords

Journal of Applied Plant Protection
Volume 4, Issue 1
December 2015
Pages 13-21

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